MiniOpticon Real-Time PCR Detection System


The MiniOpticon real-time PCR detection system comprises a compact two-color real-time detector built on the MJ Mini cycler. It is the smallest and most portable system available for real-time PCR applications and gel-free PCR analysis. Its two-color multiplexing capability allows detection of a range of fluorophores.

The MiniOpticon system uses illumination technology — samples are sequentially illuminated by a fixed array of 48 blue-green light-emitting diodes (LEDs). Each LED beam is precisely focused onto its corresponding well to ensure minimal cross talk and light scattering. Cross talk is further reduced by sequentially illuminating and detecting each well. LEDs provide a long-lived, maintenance-free excitation source. Emitted fluorescence is detected by one of two sensitive, filtered photodiodes. This innovative no-moving-parts design allows accurate detection in a compact and robust package.

The MJ Mini cycler offers precise thermal control and a temperature gradient feature that permits simultaneous incubation at 8 different temperatures, optimizing reactions in a single run.

Key Features

  • 2-color real-time PCR detection
  • 48-well capacity
  • Weight of just 15 lb and a compact footprint (18 x 32 x 33 cm W x D x H)
  • Ability to run up to 4 instruments from a single computer